Fig. 2.

The effect of dimethylamiloride (DMA; 100 μM) and ouabain (1 μM) on the rate of cytoplasmic pH recovery after ammonium chloride removal in the presence and in the absence of bicarbonate. DMA was introduced after establishment of a steady baseline pH but before the addition of ammonium chloride. Ouabain was added 5 min after addition of DMA. DMA and ouabain were present throughout the rest of the experiment. Values are means ± SE of results from 3–37 independent experiments. **P < 0.05, ***P < 0.001, significant difference from respective control. ###P < 0.001, significant difference from 1 μM ouabain treatment.