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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Prostate. 2008 Jun 1;68(8):893–901. doi: 10.1002/pros.20762

Figure 5.

Figure 5

Characterization of cell lines. A. Flt-1 expression in PE-L-1 cells. RNA isolated from PE-L-1 cells or MLE-ßgal cells was analyzed by RT-PCR with primers specific for flt-1. As a control, the same samples were analyzed by RT-PCR with primers specific for ß-actin. B. VEGF expression in transfected MLE-ßgal cells. RNA isolated from MLE-ßgal cells transfected with an expression vector for VEGF-A (MLEVEGF) or with the vector alone (MLE) was analyzed by RT-PCR with primers specific for VEGF or ß-actin. C. VEGF protein expression in transfected MLE-ßgal cells. Conditioned medium from confluent cultures of MLE-ßgal cells transfected with an expression vector for VEGF-A (MLE-VEGF) or with the vector alone (MLE) was concentrated 20-fold, run on an 8% polyacrylamide gel, and blotted onto nitrocellulose. Blots were probed with antibodies to VEGF-A.