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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: J Dent Res. 2008 Aug;87(8):777–781. doi: 10.1177/154405910808700812

Table 1.

Kinetic Constantsa for Clarithromycin Transport by SCC-25 Cells and Gingival Fibroblasts

Cell type Km (μg/ml) Vmax (μg/min/106) Cellular/Extracellular concentration ratio
SCC-25 cell line 227 ± 20 b 381 ± 29.5 b 3.3 ± 0.28 c
gingival fibroblasts 78.4 ± 7.2 b 264 ± 32.1 b 37.9 ± 2.6 c
a

Km (Michaelis constant) and Vmax (maximum transport velocity) were determined by Lineweaver-Burk analysis of transport activity assayed in the presence of several different concentrations of clarithromycin (8 to 50 μg/ml) during the rapid initial phase of uptake (first 3 minutes). The Cellular/Extracellular concentration ratio was determined in medium containing 2 μg/ml clarithromycin. The data represent the mean ± SEM of four experiments with SCC-25 cells and three experiments with gingival fibroblasts.

b

Values within columns are significantly different (P < 0.05, t-test)

c

Values within columns are significantly different (P < 0.05, Mann-Whitney rank sum test)