Fig. 1.

Western blot analysis of the amounts of pyruvate dehydrogenase kinase-4 (PDK4) protein present in skeletal muscle (A) and diaphragm (B) mice fed the chow diet (Chow) and the high-fat (HF) diet for 18 wk. Mice were killed at 7:00 AM. Protein samples (50 μg) obtained from fed mice were separated on a 15% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane. Western blotting was carried out by a standard method. Data were obtained with 4 wild-type mice in each group. *P < 0.0001 relative to mice fed Chow. VDAC1α, voltage-dependent anion channel-1α.