Table 1.

General characteristics and ²H labeling of fed vs. acutely fasted rats

Study 1	Fed	Fasted
Initial body mass, g	311±4	311±3
Terminal body mass, g	367±6	345±3*
Heart mass, g	1.02±0.04	0.99±0.02
LV mass, mg	804±38	791±18
LV/tibial length, mg/cm	189±8	184±4
2H labeling body water, % enrichment	2.72±0.10	2.57±0.09
2H labeling protein-bound alanine, %enrichment
LVwhole tissue	0.37±0.02	0.36±0.02
LVmitochondrial compartment	0.57±0.05	0.49±0.07
LVcytosolic compartment	0.39±0.03	0.38±0.02
Liver	1.56±0.04	0.99±0.18*
Gastrocnemius	0.21±0.02	0.14±0.02†
Protein synthesis rate, %newly made/h
LVwhole tissue	0.62±0.04	0.58±0.02
LVmitochondrial compartment	0.98±0.14	0.81±0.13
LVcytosolic compartment	0.65±0.04	0.62±0.04
Liver	2.65±0.10	1.96±0.06*
Gastrocnemius	0.35±0.04	0.23±0.03†

Body mass was determined as well as that of various tissues. LV, left ventricle. ²H labeling used to calculate protein synthesis is shown. In all cases, data are shown as means ± SE; n = 8 per group.

^*P < 0.01,

^†P < 0.05 vs. fed.