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Western analysis of the effects of physiological concentrations of glucagon or insulin on the ratio of phosphorylated Akt (p-Akt) to total Akt in the absence and presence of the PI3K inhibitor LY-294002 (10 μM) in perfused working rat hearts. Bars depict means ± SE of 3 determinations. *Significant difference from a normalized ratio of 1.0 in the untreated group (P < 0.05).
