Figure 4.
BRCA2 forms a complex with p53 in vivo. (A) Cell lysates (40 μg of total protein; lanes 1–3) and p53 immunoprecipitates (4 mg of total protein; lanes 4–6) were resolved by 6% SDS/PAGE and immunoblotted with anti-C15. (B) Cell lysates (lanes 1–3) were separated by 10% SDS/PAGE and immunoblotted with anti-p53 PAb421. Anti-C15 immunoprecipitates from [35S]-methionine-labeled cells were eluted and reprecipitated with anti-p53 DO-1. The double immunoprecipitates were resolved by 10% SDS/PAGE and subjected to fluorography (lanes 4–6).