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. 1997 Nov 11;94(23):12354–12359. doi: 10.1073/pnas.94.23.12354

Figure 1.

Figure 1

Levels of the precursor and nuclear forms of SREBP-1 and SREBP-2 in membranes and nuclear extracts from livers of male hamsters fed the indicated diet for 10 days. Each lane contained a pooled sample from livers of four hamsters that were fed normal chow (lanes 1 and 6), 0.1% cholesterol (lanes 2 and 7), 0.5% cholesterol (lanes 3 and 8), 1% cholesterol (lanes 4 and 9), or lovastatin/Colestipol (lanes 5 and 10) (same animals as in Fig. 5). Aliquots (30 μg protein) of membranes (Upper) and nuclear extracts (Lower) were subjected to SDS/PAGE. Immunoblot analysis was performed with either 5 μg/ml of mouse mAb (IgG-2A4) against amino acids 301–407 of human SREBP-1 (lanes 1–5) or 1:4,000 dilution of rabbit IgG against amino acids 32–250 of hamster SREBP-2 (lanes 6–10). Bound antibodies were visualized with the ECL system. Blots were exposed to film for 60 sec (lanes 1–5) or 120 sec (lanes 6–10). P, precursor form of SREBP; N, nuclear form of cleaved SREBP. X denotes crossreactive proteins whose levels did not change with these dietary manipulations. L/C, lovastatin/Colestipol.