Figure 2.

Characterization of eIF6 cDNA encoding eIF6. (A) The ATG assigned as the translation start codon (numbered +1) was found in-frame with the partial amino acid sequence of a CNBr peptide of purified eIF6 (underlined). There is no other ATG in the 5′-untranslated region. The translation stop codon TGA at nucleotide position +736 is marked with an asterisk. The position of the transcription initiation site of eIF6 mRNA determined from primer extension analysis of Fig. 3B is shown by a downward arrow (↓) at position −78 of the eIF6 cDNA. [The length of the poly(A) tail at the 3′ end of the cDNA varies between 20 and 30 in different clones and is indicated by (A)n.] (B) Restriction map of eIF6 clones obtained from human cDNA libraries. a, the 1.045-kb partial HeLa cDNA clone (clone a); b, the 1.096-kb complete human skeletal muscle cDNA clone (clone b). The black bar indicates the HeLa cell cDNA fragment used as a probe for screening λgt 10 skeletal muscle library as well as for Northern analysis in Fig. 3.