Fig. 3.
Effects of nephrin coexpression on whole cell currents recorded from HEK-293T cells expressing various Slo1 splice variants. Recording electrodes contained 5 μM free Ca2+ to allow for activation of BKCa channels by families of depolarizing voltage steps (shown in A) from a holding potential of −60 mV. A: representative traces illustrating a marked increase of currents in cells coexpressing nephrin with Slo1VEDEC. Nephrin had no effect in cells expressing the other isoforms, which exhibited large currents even in the absence nephrin. B: quantification of results from many cells. Data are means ± SE (n > 15 cells in each group). The only significant effect of nephrin was in cells expressing Slo1VEDEC (P < 0.05).