Fig. 4.

Nephrin does not substantially alter gating properties of BK_Ca channels. A: representative traces of whole cell currents evoked by depolarizing steps to +80 mV from a holding potential of −60 mV in HEK-293T cells expressing different Slo1 isoforms, as indicated, in the absence (solid traces) or presence (shaded traces) of nephrin. Amplitudes of traces are scaled to allow comparison of their time courses. Recording electrodes contained 5 μM Ca²⁺. B: activation curves constructed from HEK-293T cells expressing Slo1_VEDEC in the presence and absence of nephrin. Nephrin markedly increased mean current at all membrane potentials but did not produce any substantial effects on the voltage dependence of activation.