Table 1.

Nephrin coexpression and voltage dependence in HEK-293T cells expressing Slo1 splice variants

Splice Variant	Activation Time Constant, ms
	Control	Nephrin
Slo1VEDEC	6.41±0.31	8.60±0.84
Slo1QEERL	7.63±0.46	7.62±0.56
Slo1EMVYR	6.25±0.54	6.75±0.51

Coexpression of nephrin did not affect the activation kinetics of currents evoked by a depolarizing step pulse in HEK-293T cells expressing various Slo1 splice variants. Time constants were calculated from single-exponential fits to the rising phase of currents evoked by a depolarizing step to +80 mV from a holding potential of −60 mV. Examples of the types of current traces used for these analyses are shown in Fig. 4A. Data are means ± SE of the time constant derived from 10 cells in each group. None of these data are statistically significant at the 0.05 level as determined using Student's unpaired t-test.