Fig. 1.
Expression of mutant aquaporin-2 (AQP2) protein in LLC-PK1 cells. LLC-PK cells were transfected with various AQP2 constructs including S261A, S261D, S256A/S261A, S256A/S261D, S256D/S261A, and S256D/S261D. Cell lysates were obtained from stably transfected cells and subjected to SDS-PAGE and immunoblotting with anti-AQP2 antibody. Arrows indicate AQP2 and actin, respectively. Molecular weight markers (WM) are indicated on the left. The expression levels of the various AQP2 constructs were similar in all cell lines examined.