Fig. 3.

Effect of PP1 on phosphorylation of Src, Akt, and ERK1/2 in RPTC. RPTC were cultured for 48 h and then treated with various concentrations of PP1 or 20 μM PP3 for 1 h. A: cell lysates were prepared and subjected to immunoblot analysis using antibodies to phospho-Akt, Akt, phospho-ERK1/2, or ERK1/2 or using the pY 416 phosphospecific antibody to Src family kinases (SFKs). Representative immunoblots from 3 or more experiments are shown. B: immunoblotted phospho-SFKs were quantified by densitometry, and data are expressed as percentage of expression level relative to control.