Fig. 6.

Effect of specific siRNA on Fyn, Lyn, or Src expression. RPTC were transfected with scrambled siRNA or siRNA specific to Fyn, Lyn, or Src and then cultured for 48 h in DMEM with 0.5% FBS. Cells were harvested and immunoblot analysis was carried out using antibodies against Fyn, Lyn, Src, or actin. Representative immunoblots from 3 experiments are shown (A, C, E). Expression levels of Fyn, Lyn, or Src were quantified by densitometry, and expressed as the percentage of individual protein levels in RPTC treated with the scrambled siRNA (B, D, F).