Fig. 8.

Effect of Src siRNA on phosphorylation of Akt, or ERK1/2, and expression of cyclin D1, p27, or p57. RPTC were transfected with scrambled siRNA or Src siRNA and cultured for 48 h in DMEM with 0.5% FBS. Then, cells were incubated in the DMEM with 5% FBS for an additional 48 h, and then cell lysates were prepared and subjected to immunoblot analysis using antibodies to Akt, phospho-Akt, cyclin D1, p27, p57, or actin. A: representative immunoblots from 3 or more experiments. Expression levels of cyclin D1 (B), p27 (C), or p57 (D) were quantified by densitometry and expressed as means ± SD (n = 3).