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Effect of PLZF on the expression of epithelial Na channel (ENaC) subunit mRNAs. M1/TETOn-hPLZF cells were grown as above, and hPLZF expression induced with 500 ng/ml dox for 4–48 hrs. Cells were then lysed and the levels of alpha (upper panel), beta (middle panel) and gamma (lower panel). ENaC mRNA determined as described in the materials and methods. *, P < 0.05; **, P < 0.01; n = 5 for each time point.
