. 2008 May 21;295(1):F272–F282. doi: 10.1152/ajprenal.00200.2007

Table 1.

Primers used for RT-PCR assays

	Forward Primers	Reverse Primers	Product Size, bp	Ref. Sequence
ENaC-α
1st	`TCCCAGGATTGGATCTTCGAG`	`ACCACAGAGAGCACGGACGA`	209
2nd	`GAGATGCTGTCCTTGCAGAAC`	`GGACGAGCCAAACCACAGG`	177	NM031548
ENaC-β
1st	`GACCATCTCCATGGCTGACTG`	`GGTTAGAGAGCAGCCACACG`	200
2nd	`CGAGGCCTCTGAGGATTG`	`CAGCCACACGATATTGTTGG`	163	NM012648
ENaC-γ₁
1st	`TCTGAGGCTTCCGAGAAATG`	`CCACCAAAGTTGGACAGGAG`	182
2nd	`ATGGTTGCTGAATGTTCTCAC`	`CCACCAAAGTTGGACAGGAG`	165	NM017046
ENaC-γ₂
1st	`CCCTCCTCGTCTTCTCTTTCT`	`CCCATACAAGGACAGCAAGG`	176
2nd	`CCGTCTCTGTCTCCATCAAAG`	`GCCTGTTTTGTCTCACTGTCC`	133	NM017046
β-Actin
1st	`CTCATGAAGATCCTGACCGAG`	`CTAGGAGCCAGGGCAGTAAT`	404
2nd	`CTGTGCTATGTTGCCCTAGACTTC`	`CATCCTGTCAGCAATGCCTG`	294	NM031144

ENaC, epithelial Na⁺ channel.