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. Author manuscript; available in PMC: 2009 Jul 18.

Published in final edited form as: Biochem Biophys Res Commun. 2008 May 15;372(1):97–102. doi: 10.1016/j.bbrc.2008.04.189

Fig. 1 — (A) Silencing PU.1 expression in siRNA cell lines, PU 5.7 and PU 5.9, was determined by Western blot with α-PU.1 antibody (top panel) and α-actin antibody for internal controls (bottom panel). (B) Potential cross-silencing was examined in PU 5.7 by Western blot with α-ETS ½ antibodies. (C) TLR4 mRNA expression was examined by semi-quantitative RT-PCR of PU 5.7 (left panel) and PU 5.9 (right panel). Included was PCR for TLR4 without RT reaction (-RT) to exclude genomic DNA contamination. β-actin as internal controls was similarly analyzed.

Fig. 1 — (A) Silencing PU.1 expression in siRNA cell lines, PU 5.7 and PU 5.9, was determined by Western blot with α-PU.1 antibody (top panel) and α-actin antibody for internal controls (bottom panel). (B) Potential cross-silencing was examined in PU 5.7 by Western blot with α-ETS ½ antibodies. (C) TLR4 mRNA expression was examined by semi-quantitative RT-PCR of PU 5.7 (left panel) and PU 5.9 (right panel). Included was PCR for TLR4 without RT reaction (-RT) to exclude genomic DNA contamination. β-actin as internal controls was similarly analyzed.