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. 2008 Aug 5;6(8):e191. doi: 10.1371/journal.pbio.0060191

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© 2008 Ahn et al.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Analyses of the thymus using an antibody against β-gal in newborns from a TDLacZ mouse heterozygote (Δ/+) and a wild type (+/+). Hematoxylin staining is shown on the right.

(B) β-gal activity of the thymus from 8-wk-old homozygous knock-in mouse (Δ/Δ) at 10× magnification. Cortical and medullary areas are indicated, and the boundary between LacZ stained and unstrained areas is artificially marked as a dotted line for better visualization.

(C) Flow cytometric analysis of TSCOT-expressing cells using the markers in total thymic CD45⁻ stromal cells. The profiles of cortical marker CDR1 and medullary marker UEA-1. Defined cTEC, mTEC, and nonTEC are gated. Each gated population is shown as TSCOT and UEA-1 levels on the right. Fraction of TSCOT⁺ cells are shown in percentages.

(D) Message expression of TSCOT, FoxN1, AIRE, and GAPDH by sorted thymic compartments according to CD45/MHCII status using RT-PCR (30 cycles). The cells were isolated either from newborns or 6-wk-old thymuses, and the cell surface markers used for sorting are shown on top.