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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Dev Biol. 2007 Oct 5;312(2):484–500. doi: 10.1016/j.ydbio.2007.09.052

Figure 2. Construction of K14-DN-Clim mice.

Figure 2

(A) The upper panel is a schematic of the domain structure of Clims, showing the dimerization domain (DD) and the LIM-interacting domain (LID). The lower panel shows the dominant negative form of Clim (DN-Clim) containing the LID. (B) A GAL-luciferase plasmid was transiently cotransfected into HEK293T cells with the indicated combinations of expression plasmids encoding GAL-LMO4 fusion protein, VP16-Clim2 fusion protein, and DN-Clim. We determined relative luciferase activity 40h after the transfection. The results represent the mean and SEM from at least three different experiments. (C) A schematic of the transgene showing Myc-tagged (MT) DN-Clim containing a nuclear localization signal (NLS) under control of the K14 promoter. (D-E) Immunostaining for DN-Clim expression in corneal epithelium, including limbal epithelium, at P0 and P29. (F) Immunostaining for DN-Clim expression in transgenic skin. (G) Co-immunoprecipitation of DN-Clim and LMO4. The panel shows a Western blot with Myc-tag antibody after immunoprecipitaition with an LMO4 antibody. Original magnification: E and G, 100X; F, 40X.