Figure 8.
Semi-quantitation of colocalization. (A) Counting of stress granules (SGs) in CA1. Left, the number of SGs per cell (mean +/- standard deviation, n = 5-6 per group) in CA1 of experimental groups as indicated. Yellow channels (right, showing representative samples) were constructed from orthographic projections of 3.5 micron z-stacks acquired under 63X oil immersion of TIA-1/poly(A) staining. ANOVA p = 0.468. (B) Left, integrated density (arbitrary units) of colocalization of poly(A) and S6 in CA1 and CA3 (mean +/- standard deviation) of experimental groups as indicated. ANOVA p was 0.070 for CA1, and 0.015 for CA3. *, post hoc p < 0.05 compared to NIC group. Right, representative yellow channels from CA3 samples.