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. 2008 Aug 13;3(8):e2973. doi: 10.1371/journal.pone.0002973

Figure 4. The relative level of mRNA of hrpL and hrpS in Dickeya dadantii 3937 (Ech3937) and rsmB mutant Ech148 grown for 12 h in minimal medium.

Figure 4

The amount of mRNA was determined by qRT-PCR. Three replicates were used in this experiment. The p-value was calculated using Relative Expression Software Tool as described by Pfaffl et al. [41]. There is no significant difference between Ech3937 and Ech148 for gene hrpS with the p>0.3, but gene expression of hrpL is significantly different between Ech3937 and Ech148 with p<0.008.