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. Author manuscript; available in PMC: 2009 Jun 5.
Published in final edited form as: Brain Res. 2008 Apr 1;1213:1–11. doi: 10.1016/j.brainres.2008.03.034

Figure 4.

Figure 4

Evidence for activation of calpain, a protease associated with necrotic neurodegeneration, following repairs employing cardiopulmonary bypass with DHCA. The intact α-spectrin and its proteolytic fragments detectable in CSF following DHCA were characterized for case 5 by Western blot analysis using either an antibody reactive with all forms of α-spectrin (>250 kDa), or cleavage site-specific antibodies reactive with the NH2-or COOH-terminal fragments (~150 kDa each) generated by calpain proteolysis. Note that there is a large time-dependent increase in CSF levels of the two α-spectrin fragments derived from calpain cleavage.