FIG. 3.

Cortical and medullar renal sodium transporter abundance profile in sodium-rich diet. Effect of dietary NaCl loading for 10 days on major renal sodium transporter protein abundance in diabetic mother offspring and control mother offspring kidney. Semiquantitative immunoblots of membrane fractions from the cortex (A) and outer medulla (inner stripe) (C) obtained from diabetic mother offspring and their control mother offspring. For each blot, each lane was loaded with a homogenate from a different rat (n = 6 for both control mother offspring and diabetic mother offspring in cortex; n = 5 for control mother offspring and n = 6 for diabetic mother offspring in the medulla). Densitometric analyses revealed a significant increase in β- and γ-EnaC and Na/K ATPase abundance in diabetic mother offspring compared with control mother offspring in the cortex (B) and a significant decrease in BSC1 in the outer medulla (D). The results are expressed as the percentage of the control values. Bars represent means ± SE (unpaired t test between diabetic mother offspring and control mother offspring groups, *P < 0.05).