FIG. 1.

IL-8 and VEGF expression and release by human pancreatic duct cell preparations during culture. Human pancreatic duct cells were cultured for up to 15 days as described in research design and methods. Cell samples were taken for RNA isolation at indicated times. A: Expression levels of IL-8 and VEGF were measured by real-time PCR and normalized to HPRT. Medium was sampled at the indicated times and assayed for IL-8 and VEGF. B: Values are expressed as picograms per milliliter released by 10³ cells over 24 h. Values are means of four independent experiments ± SE. P vs. start (day 0 or day 1): *P ≤ 0.05.