FIG. 2.

TSLP-DCs induce antigen-specific CD8⁺ T-cell differentiation. A: Proliferative response of naïve 8.3-CD8⁺ T-cells (2 × 10⁴ lymphocytes/well) to 1 μg/ml NRP-A7 peptide–pulsed iDCs or LPS- or TSLP-irradiated dendritic cells (5 × 10³ cells/well), as indicated. Background responses against the negative control peptide TUM were subtracted. Quantification of IL-2 (B) and IFN-γ (C) secreted by naïve 8.3-CD8⁺ T-cells cultured under these conditions. D: Cytotoxic activity of BM-DC–activated 8.3-CD8⁺ T-cells against 1 μg/ml NRP-A7–or 1 μg/ml TUM-pulsed CFSE-labeled RAMS-K^d target cells. The percentage of killing was determined by subtracting nonspecific (TUM) from specific (NRP-A7) killing. Data are representative of two to three independent experiments and are the average ± SD.