FIG. 1.

Protein expression of IKKβ and IκBα. A: Protein expression of IKKβ was unaltered in cells exposed to a scrambled siRNA sequence, a 2-h incubation with TNF-α (lanes 1–3). siRNA against IKKβ reduced protein expression by 73% (lanes 4 and 5). Glyceraldehyde-3-phosphate dehydrogenase (GADPH) expression was determined to control for equal loading. B: IκBα was measured after siRNA-mediated depletion of IKKβ. Results are means ± SE. *P < 0.05 vs. transfected scrambled siRNA (Scr) control (n = 4).