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GSK-3β and AS160 phosphorylation. Representative immunoblot showing pGSK3β Ser9 (A) and Akt substrate AS160 (B) phosphorylation in response to insulin or TNF-α under basal conditions (□) and after insulin stimulation (▪). Graphs are summarized data (mean ± SE). *P < 0.05 vs. respective basal. †P < 0.05 vs. TNF-α–stimulated untransfected cells. #P < 0.05 vs. control basal (GSK3β, n = 3; AS160, n = 4).
