FIG. 5.

Effect of iNOS gene disruption and rosiglitazone treatment on AMPK activation and energy expenditure in high-fat–fed mice. Phosphorylation of AMP-activated kinase (P-AMPK) and ACC (P-ACC) and total expression were evaluated in quadriceps muscle (A), liver (B), and WAT (C) by Western blotting. Data are expressed relative to untreated chow-fed iNOS^+/+ mice values (dashed lines) analyzed on the same gels. D: Whole-body energy expenditure (top) and respiratory quotient (bottom) were measured by indirect calorimetry. Means ± SE of 4–8 mice per group. *P < 0.05 vs. respective untreated mice, #P < 0.05 vs. respective iNOS^+/+ mice, †P < 0.05 vs. untreated high-fat–fed iNOS^+/+ mice.