TABLE 2.
Intracellular Ca2+ and mechanical stress (for 1.5 mmol/l [Ca2+]o, 5 Hz, 37°C)
| Control rats | Diabetic rats | |
|---|---|---|
| Peak 340/380 ratio | 1.1 ± 0.1 (10) | 1.2 ± 0.2 (7) |
| Resting 340/380 ratio | 0.41 ± 0.05 (10) | 0.45 ± 0.1 (7) |
| Time to peak fluorescence (ms) | 34 ± 1 (9) | 39 ± 2 (7)* |
| Time constant of fluorescence decay (ms) | 49 ± 3 (9) | 61 ± 3 (7)† |
| Maximum rate of rise of 340/380 ratio (s−1) | 24 ± 3 (9) | 21 ± 3 (7) |
| Peak stress (mN/mm2) | 17 ± 2 (9) | 10 ± 1 (8)† |
| Resting stress (mN/mm2) | 5 ± 1 (9) | 4 ± 1 (8) |
| Time to peak stress (ms) | 67 ± 2 (9) | 77 ± 3 (9)* |
| Time to 50% relaxation (ms) | 32 ± 1 (9) | 42 ± 4 (9)† |
| Time to 90% relaxation (ms) | 56 ± 3 (9) | 76 ± 7 (9)† |
| Maximum rate of rise of stress (mN/mm2 per s) | 350 ± 60 (9) | 180 ± 20 (9)† |
Data are means ± SEM (n).
*
P < 0.01 and
†
P < 0.05, Student's t test.