Table 1.
Primers for real time RT-PCR
| Gene | Primer sequences (5′ to 3′) | Product size
(bp) |
Amplification efficiency |
|---|---|---|---|
| XIST | F: GAAGAGATGCTCCAGGCCAAT | 87 | 1.92 |
| R: AGGTGTTGCTGGCTGATGCT | |||
|
| |||
| HPRT11 | F: CGTCTTGCTCGAGATGTGATG | 98 | 1.91 |
| R: TCCAGCAGGTCAGCAAAGAA | |||
|
| |||
| G6PD | F: CCTCCTGCAGATGCTGTGTCT | 112 | 1.91 |
| R: CGCCTGCACCTCTGAGATG | |||
|
| |||
| ARAF1 | F: CGGGATGGCATGAGTGTCTAC | 108 | 1.98 |
| R: GACTGTCTTTCGCCCCTTGA | |||
|
| |||
| COL4A1 | F: CAGCAACGAACCCCAGAAAT | 120 | 1.89 |
| R: CAGCAAGAAGAGGCCAACAAG | |||
|
| |||
| 18S rRNA | F: GCCCGAAGCGTTTACTTTGA | 93 | 1.96 |
| R: CCGCGGTCCTATTCCATTATT | |||
F = Forward primer, R = Reverse primer.