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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Exp Cell Res. 2008 May 10;314(11-12):2266–2278. doi: 10.1016/j.yexcr.2008.04.018

Figure 6.

Figure 6

A. Ectopic expression of cyclin D3 dramatically increases fusion of DM1 cells during differentiation. Empty vector and pAdTrack-cyclin D3 plasmid were transfected into DM1 cells using Amaxa Transfector protocol. Fusion media was added on the next day after transfections and nuclei were stained with DAPI at day 3 after addition of fusion media. Three fields (numbered on the left) are shown for the control (vector) and cyclin D3 transfections. The field #3 of cyclin D3 transfection shows an example of cells containing more than 20 nuclei. These cells represent approximately 10% of green cells in cyclin D3 transfections and are not detectable in control DM1 cells. B. Bar graphs show a summary of three independent experiments examining the efficiency of DM1 myoblast fusion after ectopic expression of cyclin D3. Percentage of cells containing 1–3, 4–6 and more than 6 nuclei per cell was calculated. 200–300 transfected cells were used for these calculations. C. Diagram showing a hypothesis for the pathways which regulate CUGBP1 activity in normal myogenesis. These pathways are altered in DM1 cells and are involved in the development of DM1 pathology (see text).