Fig. 4.

Hepatic SCF and c-kit mRNA expression after acetaminophen treatment. C57BL/6 mice were treated with increasing doses of acetaminophen (250, 375, or 500 mg/kg) and were killed at 6, 16, 24, 48, and 72 h after acetaminophen dosing. Liver samples were obtained, mRNA was extracted, and real-time PCR was performed to determine c-kit (A) and SCF (B) expression. SCF and c-kit mRNA levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels for the same sample. C (magnification ×20) and D (magnification ×40) show immunohistochemical staining for hepatic c-kit expression at 48 h after a 375 mg/kg dose of acetaminophen. E (magnification ×20) and F (magnification ×40) show immunohistochemical staining for SCF expression at 12 h after a 375 mg/kg dose of acetaminophen.