Fig. 5.

Gene expression analysis of small intestine, liver, and kidney in Ostα−/− animals. Total RNA of these tissues was isolated from Ostα+/+ and Ostα−/− female mice that had been fed ad libitum, and mRNA levels were analyzed via real time RT-PCR. mRNA expression levels in the small intestine (A), liver (B), and kidney (C) are functionally categorized into uptake, regulation, metabolism (Met), and excretion groupings and reported relative to the expression in Ostα+/+ animals. All samples were analyzed in triplicate, and values are means ± SE, n = 4 experiments. *Statistically significant from Ostα+/+ animals, P < 0.05; BD, below detection limit; Ntcp, sodium-taurocholate cotransporting polypeptide; Asbt, apical sodium-bile acid transporter; Shp1, small heterodimer partner 1; Fgf15, fibroblast growth factor 15; Fxr, farnesoid X receptor; Cyp7a1, cholesterol 7α-hydroxylase; Bsep, bile salt export pump; Mrp, multidrug resistance-associated protein.