Fig. 2.

Expression of ubiquitinating enzymes is upregulated in CVB3-infected mouse heart. A/J mice were infected with CVB3 or sham infected with PBS as described in Fig. 1. Nine days after infection, hearts were collected. A: Western blot was performed with anti-E1A/E1B, anti-UbcH7, anti-E6-AP, and anti-GAPDH (loading control) antibodies. Levels of expression were quantitated by densitometric analysis with the NIH ImageJ 1.37 program and normalized to GAPDH expression. Data are means ± SE (sham-infected group: n = 7; CVB3 group: n = 6). *P < 0.05 vs. sham infection; NS, no significant difference vs. sham infection. B: immunohistochemical staining for ubiquitin-activating enzyme E1A/E1B (red) was carried out as described in materials and methods. Nuclei were counterstained with hematoxylin (blue). Scale bar, 50 μm.