Fig. 5.

Effects of TGRL and TGRL lipolysis products on reactive oxygen species (ROS) production in HAEC. HAEC were preincubated for with fluorescence probe 2′,7′-dichlorofluorescein diacetate (DCFDA, 10 μM) or dihydroethidium (DHE, 10 μg/ml), followed by incubation with TGRL (456 μmol/l triglyceride) without or with LpL (2 U/ml) and NADPH oxidase and CYPC2C9 inhibitors apocynin (100 μM), diphenyleneiodonium (DPI, 50 μM), or sulfaphenazole (SP, 10 μM) for 2 h. The florescence intensity of cells was measured with a fluorescence microplate reader. Florescence distribution of DCFDA or DHE oxidation was expressed as fluorescence units. *P < 0.05 compared with TGRL-treated cells; **P < 0.05 compared with TGRL + LpL-treated cells. DCF, dichlorofluorescein.