Fig. 2.

A: Western blot analysis showing the expression of α-actin in primary cultured vascular smooth muscle cells (VSMC) prepared from mice mesenteric resistance arteries and aorta (top) and Von Willebrand factor (bottom); data are representative of n = 5. B: Western blot analysis and cumulative data of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 mitogen-activated protein (MAP) kinase in response to acute stimulation of VSMC with ANG II (100 nM) or high glucose (HG, 22 mM). *P < 0.05, statistically significant differences between control (CTR) vs. ANG II and CTR vs. HG; n = 6 for each experiment. C: immunoprecipitation/Western blot analysis and cumulative data of collagen type 1 in the conditioned media of cultured VSMC stimulated for 48 h with ANG II or HG. *P < 0.05, CTR vs. ANG II and CTR vs. HG; n = 6. D: immunostaining showing collagen type 1 network formation on VSMC, growth on cover slip, stimulated with ANG II or HG for 48 h. Data are representative of n = 5.