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Chronic treatment with insulin induces specific upregulation of PI3-kinase-δ isoform. Cells were treated without or with 100 nM insulin for 72 h. PI3-kinase protein levels were measured by Western blot analysis, using specific antibody directed against p110 subunit. Results were normalized to control and are expressed as means ± SE of 3 experiments. *P < 0.05, compared with control by ANOVA, followed by Fisher's PLSD test.
