Fig. 17.

Dominant-negative p85 regulatory subunit of PI3-kinase rescues PTP1B activity in insulin-treated cells. Cells were treated for 24 h with adenovirus expressing PTP1B, followed by infection with control adenovirus expressing EGFP or with adenovirus expressing dominant negative p85 (deletion mutant) adapter subunit (Δp85) for 48 h at MOI of 10–15. A: representative Western blot for expression levels of Δp85 (top) as well as PTP1B levels in immunoprecipitation experiments. B: summary of means ± SE of 3 independent experiments. *P < 0.05, compared with control virus treatment, followed by specific IP, by Student's t-test.