Fig. 5.

Enhancement of PDGF-induced cell motility by near pathophysiological levels of insulin. Cells were preincubated for 48 h in the absence or presence of a range of insulin concentrations, followed by treatment for 24 h with 20 ng/ml PDGF in the continued absence or presence of insulin. Culture media were replaced twice a day to attenuate the potential effect of insulin degradation. Cell motility was measured by using National Institutes of Health Image software. Results are means ± SE of 3 experiments. *P < 0.05, compared with “zero insulin plus PDGF” treatment category by ANOVA, followed by Fisher's PLSD test.