Fig. 1.

Effect of hydrogen peroxide on cardiac peroxiredoxin (Prdx) 1 (Prdx1) and Prdx2. Nonreduced (A and C) and reduced (B and D) immunoblots of whole rat cardiac extracts were probed with polyclonal antibodies against Prdx1 and Prdx2. Treatment of a series of hearts with 1–10,000 μM hydrogen peroxide induced formation of disulfide dimers for Prdx1 (A lanes 4–8 and 9–13) and Prdx2 (C, lanes 4–8 and 9–13). Treatments were carried out in duplicate hearts. Dimers are absent under reducing conditions (B and D).