Fig. 4.

Quantification of formation of disulfide dimers of Prdx1 (A) and Prdx2 (B) in rat hearts after treatment with 0–10,000 μM hydrogen peroxide. Data from ≥3 independent experiments are shown as percentage of Prdx1 or Prdx2 that exists as monomer or dimer after peroxide treatment. Measurements were made from nonreducing blots, examples of which are shown in Fig. 1. C: quantitation of Prdx hyperoxidation, which is predominantly derived from Prdx1, Prdx2, Prdx3, and Prdx4, after hydrogen peroxide treatment. Values are means ± SE. Significantly different from nonperoxide (control): *P < 0.05; **P < 0.01.