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Oligomeric behavior of cardiac Prdx2 during ex vivo treatment with hydrogen peroxide. Under basal conditions, Prdx2 exists as a mixture of disulfide-bonded dimers and partially reduced decamers. Disulfide bonds are indicated by black bars. Ex vivo treatment with 100 μM hydrogen peroxide drives oxidation of decamers and reduced dimers into disulfide-bound dimers, coincident with breakdown of remaining decamers and hyperoxidation of some monomers.
