Skip to main content
. 2008 Apr 25;295(1):L38–L43. doi: 10.1152/ajplung.00280.2007

Fig. 2.

Fig. 2.

S-nitrosothiol (SNO)/nitric oxide (NO) uptake by L2 cells treated with RSNO ± LAT inhibition. A: SNO detected by triiodide reduction of NO in cell lysates via ozone chemiluminescence after incubation with 200 μM GSNO ± 50 μMd-Cys, 200 μM GSNO + 50 μMl-Cys, 200 μM GSNO + 50 μMl-Cys + 10 mMl-Leu, 200 μM GSNO + 50 μMl-Cys + 10 mM BCH (competitive inhibitors of LAT-1). Data are means ± SE representative of 3 experiments with samples analyzed in duplicate. B: DAF-FM fluorescence signal in L2 cells treated with RSNO ± LAT inhibition. Effect of 100 μMl-Cys on 200 μM GSNO induced intracellular DAF-FM signal ± 10 mM l-Leu or 10 mM BCH (competitive inhibitors of LAT-1). Values were normalized to the maximum signal. Data are means ± SE, n = 8/condition, *P < 0.001 vs. maximum.