Figure 2.

Expression cloning of i enzyme by flow cytometry. (a) Namalwa KJM-1 cells stably transfected with the WM266-4 and SW1116 cDNA libraries were stained with the anti-i antibody (Den) and subjected to three rounds of sorting with a fluorescence-activated cell sorter. The cells with strong fluorescence intensities indicated by the bars were collected and subjected to the subsequent sorting. (b) Flow cytometric analysis of the cells transfected with the empty vector pAMo (Top), a plasmid 16-2-12 harboring iGnT cDNA (Middle), or an unrelated plasmid 16-2-31 (Bottom) after staining with the anti-i antibody (solid lines) or PBS (dotted lines).