Fig. 3.

Histological appearance of bladder tissue 48 h after 1st (A) or 3rd (E) intravesical instillation of saline, 4 (B), 48 (C), and 72 (D) h after acrolein induction of acute cystitis, and 4 (F), 48 (G), and 96 (H) h after acrolein induction of subacute cystitis. Edema and some loss of urothelium were observed 4 h after acute cystitis (B). At 4 h after subacute cystitis, edema was present, but urothelium appeared intact (F). Edema, inflammatory cell infiltration, and vasodilation were observed 48 and 72 h after acute (C and D, respectively) or 4, 48, and 72 h after subacute (F, G, and H, respectively) acrolein-induced cystitis. L, lumen; D, detrusor. Original magnification ×20 and ×100. Scale bar, 100 μm.