Fig. 4.

Measurement of NGF protein content in the bladder and L₆/S₁ dorsal root ganglia (DRG) by ELISA after acute or subacute acrolein-induced cystitis. A: bladder NGF was increased 4, 48, and 72 h after acute cystitis and 4, 48, and 96 h after subacute cystitis compared with control group. B: L₆/S₁ DRG NGF protein content was increased 4 and 48 h after subacute cystitis, but not 96 h after subacute cystitis or at any time after acute cystitis. C: intravesical k252a had no effect on bladder NGF after acute cystitis, but the increase in bladder NGF 4 and 48 h after subacute cystitis was greater than that observed at these time points after acrolein alone. D: intravesical instillation of k252a prevented increased NGF protein in L₆/S₁ DRG after subacute acrolein-induced cystitis. E: intrathecal k252a had no effect on bladder NGF after acute cystitis or 48 h after subacute cystitis. F: intrathecal k252a prevented increased NGF in L₆/S₁ DRG after subacute acrolein-induced cystitis. Values are means ± SE. *P < 0.05, acute or subacute cystitis vs. saline (control). #P < 0.05, subacute cystitis in animals treated with k252a vs. subacute cystitis in animals that did not receive k252a at the same time point.