Fig. 4.

DNA methylation status of three different imprinted genes did not change with time. A: schematic diagram depicting the relative position of CpGs studied in the DMRs of Plagl1, Mest, and Peg3. B: percent methylation at CpG dinucleotides within the DMRs of Plagl1, Mest, and Peg3 in liver at different ages determined by bisulfite sequencing. There is ∼50% methylation at each site. Although there is some variability among different positions, values at each position show minimal change with age.