Fig. 2.
Protocol for part 2. Four forearm skin sites were prepared with microdialysis fibers, local cooling/heating probe holders, and laser-Doppler probes. All probes were perfused with saline for 10 min, and then sites 2, 3, and 4 were changed from saline to propranolol (PRO) and yohimbine (YOH) (site 2), BIBP-3226 (site 3), and PRO, YOH, and BIBP-3226 (site 4), respectively. After 40 min, Tloc at all four sites was raised from 33 to 40°C at 0.1°C/min. The temperature at all four sites was maintained at 40°C for 10 min. The perfusate was then changed to SNP, and Tloc was increased to 42°C to attain maximal vascular conductance levels.